Both DT and anthrax toxin have been used as prototypes for the development of novel therapeutics. A. DT was the first toxin to be exploited as an immunotoxin, and many other toxins have since been...

Both DT and anthrax toxin have been used as prototypes for the development of novel therapeutics.

A. DT was the first toxin to be exploited as an immunotoxin, and many other toxins have since been used as immunotoxins for specific purposes. Name at least two problems with the use of DT as an immunotoxin. What strategies have been employed to overcome these limitations?

B. Describe how you would design an immunotoxin based on DT that might work to cure a tumor that over expresses on its surface the ATR (anthrax toxin receptor) protein, which has an extracellular von Willebrandt factor A domain that binds to anthrax protective antigen.