Base Pairs 1,000 900 - 800 - 700 - 600 - 500 - 400 - 300 - 200 - 100 - 50 - Enzyme sets: • AB: Avrll & Bglll E: ECORI & ECORV F: Fatl • H: Hindll & HpyCH4V • KNP: Kpnl & Ncol & Pmll • X: Xbal & Xhol 1...

9. Estimate the sizes of the bands, in each of the lanes for the gel attached below. Then, for each lane, match the fragments created with the set of restriction enzymes used in the digestion of 640 bp DNA.

Base Pairs<br>1,000<br>900 -<br>800 -<br>700 -<br>600 -<br>500 -<br>400 -<br>300 -<br>200 -<br>100 -<br>50 -<br>Enzyme sets:<br>• AB: Avrll & Bglll<br>E: ECORI & ECORV<br>F: Fatl<br>• H: Hindll & HpyCH4V<br>• KNP: Kpnl & Ncol & Pmll<br>• X: Xbal & Xhol<br>1 2<br>3<br>4<br>5 6<br>M Base Pairs<br>-1,517<br>-1,200<br>-1,000<br>006-<br>-800<br>- 700<br>-600<br>-500/517<br>-400<br>-300<br>-200<br>- 100<br>-50<br>Saved<br>Normal<br>BIIIU<br>fx I D<br>B ELUT 8 E lli<br>Lane 1<br>Lane 2<br>Lane 3<br>Lane 4<br>Lane 5<br>Lane 6<br>Band 1 size<br>Band 2 size<br>Band 3 size<br>Band 4 size<br>Band 5 size<br>Restriction enzymes used<br>DNA ladder<br>Paper DNA digest<br>

Extracted text: Base Pairs 1,000 900 - 800 - 700 - 600 - 500 - 400 - 300 - 200 - 100 - 50 - Enzyme sets: • AB: Avrll & Bglll E: ECORI & ECORV F: Fatl • H: Hindll & HpyCH4V • KNP: Kpnl & Ncol & Pmll • X: Xbal & Xhol 1 2 3 4 5 6 M Base Pairs -1,517 -1,200 -1,000 006- -800 - 700 -600 -500/517 -400 -300 -200 - 100 -50 Saved Normal BIIIU fx I D B ELUT 8 E lli Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Band 1 size Band 2 size Band 3 size Band 4 size Band 5 size Restriction enzymes used DNA ladder Paper DNA digest

Jun 11, 2022

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Base Pairs 1,000 900 - 800 - 700 - 600 - 500 - 400 - 300 - 200 - 100 - 50 - Enzyme sets: • AB: Avrll & Bglll E: ECORI & ECORV F: Fatl • H: Hindll & HpyCH4V • KNP: Kpnl & Ncol & Pmll • X: Xbal & Xhol 1...

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