At the end of the activity, the student can : 1. Determine the effect of temperature on reaction rate below and above the optimum. 2. Determine the optimum temperature of the enzyme used. Objectives:...

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Extracted text: At the end of the activity, the student can : 1. Determine the effect of temperature on reaction rate below and above the optimum. 2. Determine the optimum temperature of the enzyme used. Objectives: Reagents: Saliva, 4 ml 1% starch, 4 drops iodine in KI, 4 ml benedict's reagent. Materials: Thermometer, 8 test tubes (20 ml), test tube rack, brush, holder, tripod, wire gauze, 250 ml beaker, 10 ml graduated cylinder, dropper, alcohol lamp Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 40°C , the 4th tube at 60°Cwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict's tests. (Follow procedure in activity 8).


Extracted text: Results: Tube Temperature Benedict's test Iodine test 1 Ice (0°) 2 Room Temperature 3 40°C 4 60°C 5 Boil (100°) MSTRY