At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as defined below: sucrase sucrose (designated as A) products Starting with a sucrose concentration CAo = 1.0...


At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as defined<br>below:<br>sucrase<br>sucrose (designated as A)<br>products<br>Starting with a sucrose concentration CAo = 1.0 millimol/liter and an enzyme concentration CEo<br>0.01 millimole/liter, the following kinetic data are obtained in a batch reactor (concentrations are<br>calculated from optical rotation measurements):<br>%3D<br>CA, millimole/liter<br>0.84<br>0.68<br>0.53<br>0.38<br>0.27<br>0.16<br>0.09<br>0.04<br>0.01<br>0.00<br>0.002<br>t, hr<br>1<br>2<br>4<br>7<br>8<br>9<br>10<br>11<br>Determine whether these data can be reasonably fitted by the kinetic equation of the Michaelis-Menten<br>type or<br>K3CACEO<br>-rA =<br>where CM = Michaelis Constant<br>CA + CM<br>CA<br>and ra<br>%3D<br>If the fit is reasonable, determine k3 and CM-<br>

Extracted text: At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as defined below: sucrase sucrose (designated as A) products Starting with a sucrose concentration CAo = 1.0 millimol/liter and an enzyme concentration CEo 0.01 millimole/liter, the following kinetic data are obtained in a batch reactor (concentrations are calculated from optical rotation measurements): %3D CA, millimole/liter 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.01 0.00 0.002 t, hr 1 2 4 7 8 9 10 11 Determine whether these data can be reasonably fitted by the kinetic equation of the Michaelis-Menten type or K3CACEO -rA = where CM = Michaelis Constant CA + CM CA and ra %3D If the fit is reasonable, determine k3 and CM-

Jun 11, 2022
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