At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as follows: sucrase sucrose products Starting with a sucrose concentration CAO = 1.23 millimol/liter and an...


At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as follows:<br>sucrase<br>sucrose<br>products<br>Starting with a sucrose concentration CAO = 1.23 millimol/liter and an enzyme concentration CEO = 0.04 millimol/liter, the following kinetic data are obtained in a<br>batch reactor (concentra tions calculated from optical rotation measurements):<br>CA, millimol/liter | 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.018 0.006 0.0025<br>t, hr<br>1<br>3<br>4<br>5<br>6<br>7<br>8<br>9<br>10<br>11<br>K3CACEO<br>-TA =<br>where<br>CM = Michaelis constant<br>Ca + CM<br>If the fit is reasonable, evaluate the constants k3 and CM. Solve by the integral method.<br>

Extracted text: At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as follows: sucrase sucrose products Starting with a sucrose concentration CAO = 1.23 millimol/liter and an enzyme concentration CEO = 0.04 millimol/liter, the following kinetic data are obtained in a batch reactor (concentra tions calculated from optical rotation measurements): CA, millimol/liter | 0.84 0.68 0.53 0.38 0.27 0.16 0.09 0.04 0.018 0.006 0.0025 t, hr 1 3 4 5 6 7 8 9 10 11 K3CACEO -TA = where CM = Michaelis constant Ca + CM If the fit is reasonable, evaluate the constants k3 and CM. Solve by the integral method.

Jun 07, 2022
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