Assume a cell is “flat” and round with a diameter of 3 µm. How large does a region of interest (i.e., a rectangular array of pixels) need to be in order to cover the cell? Do this calculation for the...

Assume a cell is “flat” and round with a diameter of 3 µm. How large does a region of interest (i.e., a rectangular array of pixels) need to be in order to cover the cell? Do this calculation for the CCD detectors and magnifications in Exercise 12.8. State any assumptions that you make.

Calculate the probability of one electron of dark current being detected in a pixel of a typical CCD camera during an exposure of 50 ms. For the rate at which dark current electrons are generated, look in the specifications of a typical CCD camera.