An antibody demonstrates weak reactivity at the AHG phase when using a tube method with no enhancement reagent and monospecific anti-IgG AHG reagent. When repeating the test, which of the following...

An antibody demonstrates weak reactivity at the AHG

phase when using a tube method with no enhancement

reagent and monospecific anti-IgG AHG reagent. When

repeating the test, which of the following actions may

increase the strength of the positive reactions?

a. Adding an enhancement reagent, such as LISS or PEG

b. Decreasing the incubation time from 30 minutes to

10 minutes

c. Employing the prewarm technique

d. Decreasing the incubation temperature to 18°C