All of the following are required for two loci (A and B) to be considered in linkage equilibrium, EXCEPT:
The frequency ofB on chromosomes carrying alleleA is equal to the frequency ofB on chromosomes carrying allelea.
The two loci are located very, very close together on the same chromosome.
The frequency of any chromosome haplotype can be calculated by multiplying the frequencies of the constituent alleles.
The coefficient of linkage disequilibrium (D) is equal to zero.
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