Alcohol dehydrogenase (AD) is an enzyme whichcatalyzes the reaction of its natural substrate ethanol to formacetaldehyde.The Km of AD, fromrhinoceros livers, for ethanol is 1 X 10 -3 M. This enzyme...

1. How would the kinetic plot be affected if AD were to separately catalyze methanol and
isopropanol instead of ethanol? Assume that the overall Vmax remains the same in all 3
cases.
The kinetic plot will shift to the right position for methanol and isopropanol in
comparison to ethanol.
2. How would the Km change for methanol compared to ethanol (higher, lower, the same)?
Km value for methanol would be higher than ethanol.
3. How would the Km change for isopropanol compared to ethanol (higher, lower, the
same)?
Km value for isopropanol will also be higher compared to ethanol.
4. How would the Km’s of methanol and isopropanol compare (which higher than the other
or about the same)?
Km value for methanol would be higher than isopropanol.
5. Based upon your knowledge of the mechanisms by which enzymes work, briefly
explain how you decided to place your new Km’s.
This is a renowned fact that all the enzymes cannot be termed very specific by nature in
context of their substrate. For instance; we might take Alcohol Dehydrogenase (AD) enzyme
which can react with various types of alcohols such as; methanol, ethanol, and isopropanol.
Here is must be remembered that even though AD enzyme can show reaction with all of
above...