About the technique of phage display:
MOLECULAR BIOLOGY_advanced
The Escherichia coli cell infected by the phage codifies for the optimized ligand when the phage DNA integrates in the DNA of the bacteria.
Phages are selected if they express on their surface the optimized ligand.
One selects Escherichia coli cells that are resistant to the phage infection.
More than one optimized ligand can be selected during the panning procedure.
The ligand to be selected on the surface of the phage is non-covalently linked to one of the surface proteins.
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