A) Protein PP is the receptor for the most abundant peroxisomal sorting signal. It is known that...

Question

A) Protein PP is the receptor for the most abundant peroxisomal sorting signal. It is known that residue Asn489 of the protein PP is necessary for the binding of this peroxisomal sorting signal.

Design an experiment that could demonstrate this. Remember to state:

(1) The prediction you are trying to demonstrate

(2) Your independent variable and how you are manipulating it (state what is your control and your perturbation) and

(3) Your dependent variable (state clearly which technique you are using to measure it and what you are observing and comparing to reach a conclusion)

(Max 10-12 sentences)

B) The mutation of the residue Asn 489 to Lys in PP has been found in patients with adrenoleukodystrophy, a type of hereditary disease that damages the myelin that protects the nerve cells. These patients cannot break down very long-chain fatty acids causing them to build up in the brain, nervous system and adrenal gland. How can this single mutation have such an extreme phenotype if it does not affect any of the multiple genes encoding for the enzymes involved in this metabolic pa

Assignment Q2 A) Protein PP is the receptor for the most abundant peroxisomal sorting signal. It is known that residue Asn489 of the protein PP is necessary for the binding of this peroxisomal sorting signal. Design an experiment that could demonstrate this. Remember to state: (1) The prediction you are trying to demonstrate (2) Your independent variable and how you are manipulating it (state what is your control and your perturbation) and (3) Your dependent variable (state clearly which technique you are using to measure it and what you are observing and comparing to reach a conclusion) (Max 10-12 sentences) B) The mutation of the residue Asn 489 to Lys in PP has been found in patients with adrenoleukodystrophy, a type of hereditary disease that damages the myelin that protects the nerve cells. These patients cannot break down very long-chain fatty acids causing them to build up in the brain, nervous system and adrenal gland. How can this single mutation have such an extreme phenotype if it does not affect any of the multiple genes encoding for the enzymes involved in this metabolic pathway Max 4 sentences Max 4 sentences

assignment-q2-nlxs3mfs.pdf

Dr. Sulabh · Accepted Answer

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Peroxisomal Peptide (PTS-1) Transport Mechanism
A.
1. Protein PP (PTS-1) is a peptide that binds to the PTS1 receptor (Pex5 receptor) leading to the internalization of the peptides. The process of beta-oxidation inside the peroxisomes gets activated. The experiment includes the software docking analysis of the peptide with the protein sequence of the PTS1 receptor (Pex5 receptor) along with an understanding of the interaction between the various amino acids present on the peptide and the receptor amino acids after docking. This is the prediction of the experimental setup. The peptide is a C-terminal peptide. The composition of the peptide PTS-1(PP) at the C-terminal end is given below as follows like Serine-Lysine-Leucine-COO- (Panigrahi et al.). PTS-1 peptide sequence is essential for the entry of the proteins into the peroxisomes (Miura et al.) 
2. The control peptide will be some other peptide sequence peptide like the sterol carrier protein 2 peptide docking with the Pex5 receptor (Panigrahi et al.) (Williams et al.). The independent variable in the given experiment will be the peptide sequence of the protein like the PTS-2 peptide sequence.

A) Protein PP is the receptor for the most abundant peroxisomal sorting signal. It is known that residue Asn489 of the protein PP is necessary for the binding of this peroxisomal sorting signal....

Answer To: A) Protein PP is the receptor for the most abundant peroxisomal sorting signal. It is known that...

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