A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for chloramphenicol resistance (camR). There is a restriction site inside of the penR gene that you will use...

A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for chloramphenicol resistance (camR).  There is a restriction site inside of the penR gene that you will use to move in your gene of interest.  After cutting both your vector and insert you mix them together in a test tube.  You then transform your mixture into E. coli and plate your cells on a master plate without any antibiotics.  After colonies appear, you pick and re-plate onto a penicillin containing plate and onto a chloramphenicol containing plate.

What do you expect to grow on the Master Plate?

A. E. coli with the gene of interest in your plasmid vector

B. Impossible to tell with the provided information

C. Nothing will grow

D. E. coli with the original plasmid vector

E. E. coli with no plasmid vector