A piece of DNA fragment is sequenced. You clone the the fragment, isolate the cloned DNA fragment, and set up a series of four dideoxy reaction. You then separate the products of the reaction by gel...

A piece of DNA fragment is sequenced. You clone the the fragment, isolate the cloned DNA<br>fragment, and set up a series of four dideoxy reaction. You then separate the products of the<br>reaction by gel electrophoresis and obtain the following banding patter:<br>ddATP<br>ddTTP<br>ddCTP<br>ddGTP<br>What is the base sequence of the original fragment that you were given?<br>5'-TAAGCTGA-3'<br>O 5'-ATTCGACT-3'<br>O 5'-TCAGCTTA-3'<br>O 5'-AGTCGAAT-3'<br>

Extracted text: A piece of DNA fragment is sequenced. You clone the the fragment, isolate the cloned DNA fragment, and set up a series of four dideoxy reaction. You then separate the products of the reaction by gel electrophoresis and obtain the following banding patter: ddATP ddTTP ddCTP ddGTP What is the base sequence of the original fragment that you were given? 5'-TAAGCTGA-3' O 5'-ATTCGACT-3' O 5'-TCAGCTTA-3' O 5'-AGTCGAAT-3'

Jun 10, 2022

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A piece of DNA fragment is sequenced. You clone the the fragment, isolate the cloned DNA fragment, and set up a series of four dideoxy reaction. You then separate the products of the reaction by gel...

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