A nucleic acid with a sequence 5’-AGCUUGAGCGUCCA-3’ was hydrolyzed with a strong acid. The hydrolyzate was then subjected to qualitative color reactions. Answer the following questions below.
1. Which bases will give a positive reaction (producing the expected result) with concentrated HNO3, 10% KOH, & then evaporation to dryness?
2. What is the color of the residue in (1)?
3. If the hydrolysate is treated with diphenylamine reagent, will it give a positive result?
4. The purple coloration obtain after treatment with Br2-H2O and Ba(OH)2 is due to what components in the hydrolysate?
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