A bioconversion facility uses a substrate at the initial concentration of 50 g/L. At the end of the bioconversion 90% of the substrate is converted to the product. The fermentation takes place at 30°C. The bioconversion process follows the second order kinetics. An enzyme is used as catalyst for the bioconversion at the concentration of 2 g/L and the overall substrate utilization rate constant is 0.75 day−1. The average flow rate of the feed for the bioconversion is 10,000 L/day
a. If a batch bioreactor is used for the bioconversion, how much time does the bioconversion take for each batch? What is the minimum volume of the batch reactor required for the bioconversion? b. If tubular bioreactor or PFR is used for the bioconversion, what is the minimum volume of the PFR required for the bioconversion? c. If a continuously stirred tank reactor (CSTR) is used for the bioconversion, what is the minimum volume of the CSTR required for the bioconversion?
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