A 40 m³ (working liquid volume) stirred tank is used for a batch culture of a recom- binant bacterium. In the mid-exponential phase at an air flow rate of 30 m /min, the dissolved oxygen is 15% of...


A 40 m³ (working liquid volume) stirred tank is used for a batch culture of a recom-<br>binant bacterium. In the mid-exponential phase at an air flow rate of 30 m /min, the<br>dissolved oxygen is 15% of saturation with ambient air. The outlet gas composition<br>is 16% O2, 4.5% CO,, and 5% H,O. Calculate the OUR in mmole O,/L-h. Estimate<br>Ka for the transfer of oxygen. You can assume the system is at a quasi-steady state,<br>and the air in the inlet is water-free.<br>

Extracted text: A 40 m³ (working liquid volume) stirred tank is used for a batch culture of a recom- binant bacterium. In the mid-exponential phase at an air flow rate of 30 m /min, the dissolved oxygen is 15% of saturation with ambient air. The outlet gas composition is 16% O2, 4.5% CO,, and 5% H,O. Calculate the OUR in mmole O,/L-h. Estimate Ka for the transfer of oxygen. You can assume the system is at a quasi-steady state, and the air in the inlet is water-free.

Jun 07, 2022
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