8.14 Isocitrate lyase catalyzes the following reaction: isocitrate ion → glyoxylate ion + succinate ion The rate, v, of the reaction was measured when various concentrations of isocitrate ion were...

Determine the Michaelis constant and the maximum velocity of the reaction. Using direct linear plot

Extracted text: 8.14 Isocitrate lyase catalyzes the following reaction: isocitrate ion → glyoxylate ion + succinate ion The rate, v, of the reaction was measured when various concentrations of isocitrate ion were present, and the following results were obtained at 25°C: [isocitrate]/(µmol dm-³) v/(pmol dm³s-1) 31.8 46.4 59.3 118.5 222.2 70.0 97.2 116.7 159.2 194.5 Determine the Michaelis constant and the maximum velocity of the reaction.