7. An enzyme is discovered that catalyzes the chemical reaction : SAD + HAPPY A team of motivated researchers sets out to study the enzyme, which they call happyase. They find that the kcat for...

Extracted text: 7. An enzyme is discovered that catalyzes the chemical reaction : SAD + HAPPY A team of motivated researchers sets out to study the enzyme, which they call happyase. They find that the kcat for happyase is 600 s -1 and carry out several additional experiments. When [Et] = 20 nM and [SAD] = 40 µM, the reaction velocity is 9.6 µM s-1. Calculate KM for the substrate SAD. 8. In a separate enzyme happyase experiment using [Et] = 10 nM, the reaction velocity is measured at 3 µM s-l. What is the [S] used in this experiment?