6.1 EXPECTED RESULT FOR CELL COUNT USING HAEMOCYTOMETER Table 2 Number of cell in upper grid 15 13 Grid location number Number of cell in lower grid 20 17 1 3 4 10 14 18 13 7 20 15 17 14 13 10 10 12...

Extracted text: 6.1 EXPECTED RESULT FOR CELL COUNT USING HAEMOCYTOMETER Table 2 Number of cell in upper grid 15 13 Grid location number Number of cell in lower grid 20 17 1 3 4 10 14 18 13 7 20 15 17 14 13 10 10 12 13 18 Total cells in upper and lower grid 120 120 TOTAL CELLS 240 Total grids 16 Please calculate a numbers of cell/ml from the information table 2.


Extracted text: Cell counting calculation Basic hemacytometer formula Neubauer Hemacytometer Calculation Number of cells counted X dilution factor total volume of the cells counted total volume - # primary squares x 0.1 (deptn) Cells X dilution = cells/uL squares X 0.1 This formula can be used with any counting chamber as long as one knows the dimensions for the chamber Note: 0.1 mm depth is constant in Neubauer Hemacytometer Size Volume 0.0025 mm 0.04 mm 0.0625 mm 1 mm Blue 0.25 nl Yellow 4 nl Green 6.25 nl Red 100 nl. average live cells x dilution factor volume of a square (mL) Cell density (cells/mL) = i. Example calculation Given information bellows: No of yeast cells in 14 grids (yellow grids) = 210 No cells in average = 210/14 = 15 cells Dilution factor = 1 Total volume = Area x Depth = 0.04mm? x 0.1mm (From haemocytometer) = 0.004mm3 = 4.0 x 106 ml Cells/ml = (15cells x 1) /4.0 x 106 ml %3D = 3.8 x 10° cell/ml