11 Enzyme assay A) Always involves asking a substrate B) Can be very smoke or very complex C) Always involves ultraviolet light D) A-C are incorrect 12 Isolation of chromosomal (total) DNA using a...

11 Enzyme assay

• A) Always involves asking a substrate


• B) Can be very smoke or very complex


• C) Always involves ultraviolet light


• D) A-C are incorrect

12 Isolation of chromosomal (total) DNA using a spin column

• A) Involves a filtration step to remove many different molecules


• B) Lysis is an optional step


• C) Elution is not necessary


• D) Results in a very pure protein preparation

13 Isolation of chromosomal (total) DNA using a spin column

• A) Cells must be used using an appropriate method


• B) Cell lysis is required


• C) Elution of DNA required regardless of the cell type used in the experiment


• D) A-C are correct.

14 Isolation of chromosomal (total) DNA using a spin column

• A) Washing the spin column is optional


• B) DNA must be eluted from the spin column


• C) Does not involve centrifugation


• D) Only molecules that are supposed to bind to the column do so.