1. What caused the color changes in containers A, B, C, and D? 2. What is the effect of the sprite/ baking soda mixture on the acidity and basicity of the mixture? 3. What are buffers and why is it...

Can you please help me answer guide questions, especially number 4 Thank you.

Extracted text: 1. What caused the color changes in containers A, B, C, and D? 2. What is the effect of the sprite/ baking soda mixture on the acidity and basicity of the mixture? 3. What are buffers and why is it important in biochemistry? 4. Describe the Henderson Hasselbalch equation and explain its use in making buffers.


Extracted text: Last edit was seconus ago BIUA 三== ニm A. 11 I.. 2 3 ..|. 5. ... 6. Experiment 4 pH and buffers Materials needed Red Cabbage Purified water 5 different glasses/jars Sprite (40 mL) dropper/syringe Baking soda ( 2 teaspoon) NaHCO3 Lemon juice/ calamansi juice/ vinegar (choose one) Concentrated lye ( liquid sosa) drainage cleaner Procedure: 1. Boil Red cabbage in 700 mL water. Make sure to cut the leaves into small pieces to maximize the amount of indicator in solution. This will serve as your pH indicator 2. Let the solution cool and transfer 100mL each to a jar 3. Label the containers with the proper label, A, B, C, D. Add 1 teaspoon of baking soda and 20 mL of sprite in the mixture into container B and C. Stir it well. 4. In container A, add 1 mL dropwise of lemon juice or any acid. Stir well. Observe the changes in color. 5. In container B, add 1 ml of lemon juice or any acid. Observe the changes in color. Add another 1 mL of lemon juice 6. In container D, add 1 mL of concentrated lye. Stir well. Observe the changes in color. 7. In container C, add 1 mL of concentrated lye. Stir well. Observe the changes in color Container Observations after 1 ml of substance of lemon juice is Observations after 1 ml of substance of concentrated lye is added Conclusion added A Color changed Color changed from blue to purple. No changes Color didn't change. C Color changed Color changed from blue to yellow/dark brown. D Color changed Color changed from lili