1) Calculate the rate of oxygen consumption in % oxygen consumption/ min for each substrate. 2)...

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1) Calculate the rate of oxygen consumption in % oxygen consumption/ min for each substrate. 2) Calculate the rate of oxygen consumption in uM oxygen/ min 3) Calculate the rate of oxygen consumption in uM oxygen/min/mg mitochondria Bonus question:- Calculate the total number of oxygen molecules used by the mitochondria for substrate A.

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BS2001Metabolism Workshop 1 An investigation was carried out looking at oxygen usage by mitochondria. This experiment was carried out in a enclosed chamber with an oxygen electrode (see diagram). 3.0 mL of purified mitochondria (8 mg) were placed into the sample chamber at the start of the experiment and 0.5 mL of substrate (A, B or C) was added. Finally, 500 ul of water was added to the mixture and the oxygen consumption was monitored over a set time period, see results graphs. NB: The saturation of oxygen in solution is 0.25 uM/mL Questions Calculate the rate of oxygen consumption in % oxygen consumption/ min for each substrate. Calculate the rate of oxygen consumption in uM oxygen/ min Calculate the rate of oxygen consumption in uM oxygen/min/mg mitochondria Bonus question:- Calculate the total number of oxygen molecules used by the mitochondria for substrate A. Figure 1. Rate of consumption of oxygen by mitochondria using substrate A. Figure 2. Rate of consumption of oxygen by mitochondria using substrate B. Figure 3. Rate of consumption of oxygen by mitochondria using substrate C.

BS2001Metabolism Workshop 1 An investigation was carried out looking at oxygen usage by mitochondria. This experiment was carried out in a enclosed chamber with an oxygen electrode (see diagram). 3.0 mL of purified mitochondria (8 mg) were placed into the sample chamber at the start of the experiment and 0.5 mL of substrate (A, B or C) was added. Finally, 500 ul of water was added to the mixture and the oxygen consumption was monitored over a set time period, see results graphs. NB: The saturation of oxygen in solution is 0.25 uM/mL Questions 1) Calculate the rate of oxygen consumption in % oxygen consumption/ min for each substrate. 2) Calculate the rate of oxygen consumption in uM oxygen/ min 3) Calculate the rate of oxygen consumption in uM oxygen/min/mg mitochondria Bonus question:- Calculate the total number of oxygen molecules used by the mitochondria for substrate A. Figure 1. Rate of consumption of oxygen by mitochondria using substrate A. Figure 2. Rate of consumption of oxygen by mitochondria using substrate B. Figure 3. Rate of consumption of oxygen by mitochondria using substrate C. 0 20 40 60 80 100 120 0102030405060 Time (sec) % oxygen 0 20 40 60 80 100 120 0102030405060 Time (sec) % oxygen 0 20 40 60 80 100 120 0102030405060 Time (sec) % oxygen

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David · Accepted Answer

BS2001Metabolism Workshop 1: 
1) From the graph for substrate A: 
Time(sec) 5 15 25 35 45 55 65 
%O2 consumed 0 20 40 60 90 99 99
Plotting the graph and approximating the curve to a polynomial:
The equation of the curve: 
%O2 = 10
-06t5 - 0.0003t4 + 0.0168t3 - 0.4611t2 + 7.0348t - 25.743  
Rate of consumption of O2 (%O2 /min) = 
 
  
(            )     
               = 3*10-04t4 - 0.072t3 + 3.024t2 - 55.332t + 422.088  
The rate of consumption is a function of time.
From the graph for substrate B: 
Time(sec) 5 15 25 35 45 55 65 
%O2 consumed 0 10 30 50 70 78 97
Plotting the graph and approximating the curve to a polynomial: 
y = -1E-06x5 + 0.0003x4 - 0.016x3 + 0.4592x2 - 4.602x + 13.343 
-10
0
10
20
30
40
50
60
70
80
90
0 10 20 30 40 50 60 70
%
O
xy
ge
n
 
Time(sec)
The equation of the curve: 
%O2 = 10
-06t5 - 0.0002t4 + 0.0073t3 - 0.1071t2 + 1.5246t – 5.8352  
Rate of consumption of O2 (%O2 /min) = 
 
  
(            )     
               = 3*10-4t4 - 0.048t3 + 1.314t2 - 12.852t + 91.476  
The rate of consumption is a function of time.
From the graph for substrate C: 
Time(sec) 5 15 25 35 45 55 65 
%O2 consumed 0 5 20 30 45 65 80
Plotting the graph and approximating the curve to a polynomial: 
y = -1E-06x5 + 0.0003x4 - 0.016x3 + 0.4592x2 - 4.602x + 13.343 
-10
0
10
20
30
40
50
60
70
80
90
0 10 20 30 40 50 60 70
%
O
xy
ge
n
 
Time(sec)
 The equation of the curve: 
%O2 = 5*10
-08t6 - 10-05t5 + 0.0011t4 - 0.0487t3 + 1.1016t2 - 10.274t + 29.

1) Calculate the rate of oxygen consumption in % oxygen consumption/ min for each substrate. 2) Calculate the rate of oxygen consumption in uM oxygen/ min 3) Calculate the rate of oxygen consumption...

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