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1 BIO 2133 (Dr. Montpetit) Take Home Final Exam (90 points) April 20th 8h30am to April 21st 8h30 am (Eastern Standard Time) INSTRUCTIONS: 1. Please download a copy of the exam. You have the option to use the WORD or PDF version. 2. This exam is open book. Answer ALL questions as best you can with the information on hand. 3. This test must be completed individually. Should two or more students submit identical answers or answers that constitute plagiarism in any form, all students with those answers will face allegations of academic fraud. We are not using Respondus or any other lockdown software. 4. The test is designed to be written in a 180-minute block of time; however, you have up until April 21st 8h30 (Eastern Standard Time) to submit your answers to the test. 5. You can answer questions in any way you choose/are able. This includes but is not limited to: a. Printing the exam, filling it in by hand, then scanning/photographing it. b. Answering each question by hand on a blank paper, then scanning/photographing it. *You do not need to recopy the questions to your sheet of paper but be sure to clearly indicate which question you are answering and if you must copy sequences/images/etc… to your sheet, do so very carefully to avoid making errors. c. Handwriting your answers digitally. (e.g. with an Apple pencil or stylus capable of writing in e-ink) d. Typing your responses directly into the file. 6. You may ask questions via email. They will be answered only during regular business hours (9am to 5pm). Only questions of a technical nature (i.e. – submission issues) will be answered in the final 30 minutes of the exam. Please do not expect quick answers given the size of the class. 7. Please name and save the digital file (as a PDF file- ONLY) containing your completed exam student#_BIO2133_surname.filetype (e.x - I would name my completed exam – 12345678_BIO2133_Montpetit.pdf). 8. You must submit your exam by April 21st 8h30am (EST). This is a hard deadline. a. Upload a single file (.pdf) of the entire completed exam by April 21st 8h30am (EST). b. You will receive an email confirmation of the receipt of your submission. c. I will only accept 1 submission. To submit a 2nd copy and have it considered during marking, we must give the go ahead first. d. If you experience technical difficulties resulting in you missing the submission deadline, please contact me before the deadline to let me know (
[email protected]). e. Late submissions will not be assessed and considered an absence. Helpful Hints and Tips: ▪ If completing the exam digitally, it is recommended that before beginning to write, that you name and save the document. I also recommended that you activate any automatic save function that your software may have. mailto:
[email protected] 2 ▪ If scanning/photographing your exam to digitize a handwritten file, you may find it helpful to use a free app/program such as clear scanner (for android or iPhone) or pdf merge (web based) to compile multiple pages into a single file. Please try to create a PDF file prior to the exam, in order to test out your ability to successfully create a PDF file and to avoid any issues when trying to submit your exam. Please use 12pt Times New Rom font if you type your answers. Table 1: Probability table of chi-square values Probabilities df 0.99 0.95 0.90 0.70 0.50 0.30 0.20 0.10 0.05 0.01 0.001 1 0.0001 0.004 0.016 0.15 0.46 1.07 1.64 2.71 3.84 6.64 10.83 2 0.02 0.10 0.21 0.71 1.39 2.41 3.22 4.61 5.99 9.21 13.82 3 0.12 0.35 0.58 1.42 2.37 3.67 4.64 6.25 7.82 11.35 16.27 4 0.30 0.71 1.06 2.20 3.36 4.88 5.99 7.78 9.49 13.28 18.47 5 0.55 1.15 1.61 3.00 4.35 6.06 7.29 9.24 11.07 15.09 20.52 6 0.87 1.64 2.20 3.83 5.35 7.23 8.56 10.65 12.59 16.81 22.46 7 1.24 2.17 2.83 4.67 6.35 8.38 9.80 12.02 14.07 18.48 24.32 https://play.google.com/store/apps/details?id=com.indymobileapp.document.scanner&hl=en_CA https://apps.apple.com/us/app/clearscanner-fast-scanning/id684155862 https://www.sodapdf.com/pdf-merge/ 3 Question 1 – 6 points A. Consider the following DNA sequence (coding strand) located near the middle of the coding region of a gene in Neurospora. The numbers atop the nucleotides represent the position # of a nucleotide. DNA Which of the following DNA mutations is almost certain to result in a longer than normal mRNA? You may choose more than one answer. In any event, explain your choice(s). (3 points) • G → A mutation at nucleotide #50 • A → G mutation at nucleotide #54 • G→T mutation at nucleotide #60 • None of them B. In an experiment in Neurospora, you have successfully isolated a conditional mutation that shows the following phenotypes: • At 30oC, it can synthesize DNA • At 42oC, no DNA is synthesized, and the cells die. What type of mutation is this most likely? Explain. (3 points) 5’- CCT G A G T C C G A G G G T G A A C G A G -3’ 50 55 60 65 4 Question 2 – 5 points In humans, lactase is an enzyme that plays a role in digestion of milk. The ability to digest lactose makes human lactose tolerant while the inability to digest lactose makes humans lactose intolerant. For the purposes of this scenario suppose that lactase activity typically decreases to very low levels after 3 years of life. Also, suppose there were three different types of alleles for the lactase gene: L1 – Wild type allele L2 – Allele with a gain-of-function mutation in the promoter region of the gene. L3 – Allele with a loss-of-function mutation in the coding region of the gene. Predict (that is draw) what would be lactase activity for the individual based on the provided genotype. NOTE: Use a line graph (not a bar graph) to show changes over time to demonstrate your understanding. Use graph #1 for L1L1 as your point of reference against which to relate the tendencies in the other graphs. An example of a line graph is shown in the graph for L3L3. The dotted line represents threshold activity. age 3 15 la ct as e ac ti vi ty age 3 15 age 3 15 age 3 15 la ct as e ac ti vi ty age 3 15 age 3 15 Genotype: L1L1 Genotype: L2L2 Genotype: L3L3 Genotype: L1L3 Genotype: L1L2 Genotype: L2L3 5 Question 3 – 5 points A couple is about to give birth to their first child. Neither parent is afflicted with a genetic condition. Given their age, however, the couple decided to have genetic tests done to know if their child will be afflicted with a genetic condition. Among the various tests, karyotypes were performed for each parent. The figures below show chromosomes from the father and the mother. Chromosome 2 is stained with a pink dye. A. What type of chromosomal anomaly is exhibited in the mother’s karyotype? Provide an explanation for which the mother does not show any symptoms associated with the chromosomal abnormality. (2 points) B. Given the karyotype results, the couple is concerned about the health of their child. To the couple’s surprise, the genetic counsellor says that there is a possibility that their child may not demonstrate abnormalities and grow up healthy. Explain the counsellor’s reasoning. (3 points) MotherFather 6 Question 4 – 5 points Several cells like the one represented on the right (cell labelled A) undergo a normal meiosis I and meiosis II, so that each cell produces four daughter cells. Possible daughter cells are shown below. For each question, cells labelled A-E are possible choices; none of the cells can be a choice. a. Which cell or cells are haploid? (1 point) b. Which cell or cells demonstrate a crossing over? (1 point) c. Which cell or cells appear to be in meiosis I? (1 point) d. What is the ploidy of the cell labelled A? (1 point) e. Which cell or cells show nondisjunction? (1 point) A. B. C. D. A. B. C. D. E. 7 Question 5 – 6 points In a research project, you discover a new wing phenotype in drosophila and termed it ZigZag. Results demonstrate that the ZigZag phenotype is due to a mutation. Results from crosses suggest that the ZigZag phenotype is recessive (Z-) to the wild-type phenotype (Z+). As a result of your finding, your Prof C tasks you to map this new gene in the drosophila genome. Prof C suspects that this new gene either linked to the single nucleotide polymorphic marker (SNP) 1DMC (located on chromosome 1) OR the SNP 2THC (located on chromosome 2). Using the drosophila strains available to you, describe the crosses you would conduct to map this newly discovered gene to either chromosome. Clearly indicate the genotypes of the individuals you would cross. (4 points). The following pure breeding strains of drosophila are at your disposal: . Explain how you will know on which chromosome the wing phenotype will be mapped on (2 points). 1) Z+, 1DMCA, 2THCT 2) Z-, 1DMCA, 2THCT 3) Z-, 1DMCA, 2THCC 4) Z-. 1DMCG, 2THCC 5) Z+, 1DMCA, 2THCC 8 Question 6 – 8 points Shown below is the gene map for drosophila melanogaster. In drosophila, vermillion eyes are recessive to red