0.5 104 10 102 10 1 10' 10² 103³ 10* Cot (mole × sec/L) In 1968, Britten and Kohne showed that once double-stranded DNA is dissociated into single- stranded pieces, the rate of reassociation of the...

Please help.I am stuck

Extracted text: 0.5 104 10 102 10 1 10' 10² 103³ 10* Cot (mole × sec/L) In 1968, Britten and Kohne showed that once double-stranded DNA is dissociated into single- stranded pieces, the rate of reassociation of the complementary strands can be analyzed. For these kinds of experiments, the DNA is first sheared into fragments with an average size of several hundred base pairs. The fragments are dissociated (denatured) via heating, and then the sample temperature is lowered slowly while the reassociation is monitored. During reassociation, pieces of single-stranded molecules collide at random. If the pieces are complementary, a stable double-stranded molecule is formed. If no match is made, the pieces separate and continue encountering other single-stranded pieces. This process continues until all matches are made. a. The figure below shows an ideal time course for reassociation of DNA. Co is the initial concentration of DNA single strands in moles per liter. Time (t) of the reassociation is measured in seconds. C is the concentration of single-stranded DNA remaining after some time, t, has elapsed. For simplicity, you can think about the X-axis as time elapsed. i. At time t = 0, what is the relationship of C vs. Co? ii. You can see the slope of the line changes over the span of the reaction. Describe the slope at the beginning, middle and end of the reaction using the following words: steep and shallow. iii. Explain why the slope is as you describe it at the beginning, middle and end of the reaction. Provide a molecular explanation.